Drug screen test does not require any handling or manipulation to activate the test. Just dispense urine into cup and read results in only 5 minutes. Keep sealed until ready to use. Directions for use: 1. Insert into the test cup and replace cap. 2. Wait 5 minutes before reading results. 3. If positive, send to laboratory for analysis. This drug test cup provides preliminary test results. A more specific chemical method (such as GC/MC) should be used to confirm the results. Test procedure: 1. Urinate into cup, filling the cup with urine above the minimum level line. 2. Stand the cup upright on a solid surface. 3. Wait 5 minutes. 4. Read results. 5. After test results are confirmed please dispose of the entire cup. Negative: Two lines indicate a negative result. Positive: One line indicates a positive result. Retest: No line requires a retest. See instructions inside for more details about reading results. Please note: This is only a preliminary home drug testing kit for instant results. For confirming results, check with your family physician or health care professional.